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General information

In collaboration with Uppsala University, this course qualifies for 5 hp for PhD students after separate examination.

December 3 - 6, 2012.

Location: Lisbon, Portugal

Course fee: Early registration: 1950 € plus VAT
Standard registration: 2200 € plus VAT
Late registration: 2300 € plus VAT
For university participants:
Early registration: 1100 € plus VAT
Standard registration: 1200 € plus VAT
Late registration: 1300 € plus VAT

Early registration: up to 2 months before the start of the course
Standard registration: up to 1 month before the start of the course
Late registration: less than 1 month before the start of the course 
Lecturers: Dr. Jan Ståhlberg and Dr. Per Lövkvist

Who should take this course?

This course gives the scientific background to liquid chromatography. It is specially designed for those that work with advanced method development, study chromatography scientifically or teach advanced courses in chromatography. Also those that use liquid chromatography as a preparative tool will benefit from the course.

Course Schedule

Day 1

- Introduction
- Objective of the course
- Short review of important chromatographic concepts
- Thermodynamics for chromatographers
- Thermodynamics of retention
- Molecular interactions
- Regular solution theory and solubility parameters
- Solution properties of the mobile phase
- Adsorption isotherms and surface excess

Day 2

- Mass balance equation of chromatography
- Other theoretical approaches
- Non-linear and linear chromatography
- Indirect detection
- System peaks
- Displacement chromatography
- Gradient chromatography
- Retention mechanism in reversed-phase chromatography of neutral solutes
- Charged surfaces and the Poisson-Boltzmann equation
- Retention mechanism in reversed-phase chromatography of ionic solutes
- Ion pair chromatography
- Retention mechanism of small ions in ion exchange chromatography
- Retention mechanism of proteins in ion exchange chromatography
- Size Exclusion Chromatography

Day 3

- Physical structure of column packings
- Hydrodynamics and the Reynolds number
- Poiseuille flow
- Electroosmotic flow
- Flow in packed and monolithic columns
- Diffusion
- Dispersion in tubes
- The Golay equation for dispersion in capillary columns

Day 4

- Dispersion in packed and monolithic columns
- Band broadening equations (van Deemter, Knox and others)
- Analysis of column performance (column impedance, kinetic plots etc)
- Concluding remarks and examples of how to apply physical theory to chromatography

What will you gain?

- After the course you will have a thorough understanding of all the important fundamental aspects of chromatography.
- You will have the tools that are necessary to conduct advanced method development in liquid chromatography.
- You will increase your ability to understand and critically read advanced chromatographic literature.
- If you are teaching chromatography at the university, you will have a solid theoretical background and understanding of chromatography.


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