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General information

Dates: March 19 - 22, 2012.

Location: Lisbon, Portugal

Course fee: Early registration: 1950 € plus VAT
Standard registration: 2200 € plus VAT
Late registration: 2300 € plus VAT
For university participants:
Early registration: 1100 € plus VAT
Standard registration: 1200 € plus VAT
Late registration: 1300 € plus VAT

Early registration: up to 2 months before the start of the course
Standard registration: up to 1 month before the start of the course
Late registration: less than 1 month before the start of the course 
Lecturers: Dr. Jan Ståhlberg and Dr. Per Lövkvist

Who should take this course?

The course is intended for analytical chemists with previous experience of HPLC and a good knowledge of the basic concepts of chromatography.

If you are an advanced user, e.g. responsible for the HPLC equipment in a laboratory, working with troubleshooting or developing standard HPLC methods, this course will bring your understanding of liquid chromatography to a higher level. We will provide you with new insights and tools that will be valuable for your work.

If you teach chromatography at a university you will find the course of great value when preparing lectures and laboratory experiments.

Course description

You will get a thorough understanding of:
- the fundamental chromatographic concepts (plate height, resolution, etc.)
- the retention mechanisms for different chromatographic techniques (reversed phase, ion exchange etc.)
- the various mechanism for band dispersion and how they can be controlled.

Throughout the course we emphasize how you can apply fundamental aspects to troubleshooting and method development and other aspects of your daily work. Theoretical concepts are put into a practical context through numerous experimental examples. 

Course schedule

Day 1

- Introduction to the course. 
- The role of chromatography in analytical chemistry. 
- Introduction to the principles of chromatography. 
- The basic concepts of chromatography. 
- Retention and equilibrium. 
- Introduction to band broadening. 
- Resolution. 
- Significance of different chromatographic parameters in practice.

Day 2

- Retention theory from a molecular and thermodynamic point of view. 
- Properties of stationary phases for reversed phase and ion exchange chromatography. 
- Properties of commonly used mobile phases.
- The retention of macromolecules in ion exchange chromatography. 
- The separation of macromolecules in size exclusion chromatography. 
- Physical characteristics of chromatographic columns.

Day 3

- How to apply retention theory in practice. 
- How to select the chromatographic mode. 
- How to select the column.  
- How and when to use gradient chromatography. 
- How the retention factor depends on the analyte, stationary phase and mobile phase.
- Controlling retention in method development.
- How to select the appropriate detector. 

Day 4

- Hydrodynamics in open tubes. 
- Hydrodynamics in packed columns. 
- The mechanism of band broadening in packed columns. 
   * Eddy dispersion. 
   * Diffusion. 
   * The effect of non-equilibrium. 
- Band broadening equations. 
- How the band broadening depends on the flow rate, analyte, stationary phase and mobile phase. 
- How to obtain resolution in an efficient way. 
- How to apply combined retention and band broadening theories in practice. 
- General recommendations and tips for method development. 
- General recommendations and tips for troubleshooting. 

What will you gain?

After the course you will: 
- understand how retention, band broadening and resolution can be controlled. 
- understand the basics of troubleshooting, i.e.: 
   * be able to make sound judgments concerning the properties of chromatographic methods.  
   * understand why your chromatographic methods work or why they fail. 
   * know how to critically judge chromatographic methods. 
   * know how to investigate chromatographic columns. 

- understand the basics of method development, i.e. 
   * understand how to design chromatographic methods. 
   * be able to read and understand the chromatographic literature. 
   * know how to choose suitable mobile phases. 
   * know how to choose chromatographic columns. 

- find your work more interesting and rewarding.


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